DOI 10.36461/NP.2023.67.3.014
UDC 636.085.8+619

EFFECT OF PHYTO-STEM CELLS OF MICROGREENS ON THE PHYSIOLOGICAL AND BIOCHEMICAL STATUS OF LABORATORY ANIMALS
E.G. Kulikova¹ Candidate of Biological Sciences, Associate Professor, E.V. Polyakova¹ Candidate of Biological Sciences, Associate Professor, E.V. Perunova¹ Candidate of Biological Sciences, Associate Professor, G.I. Yuskaeva² Candidate of Biological Sciences, E.K Kistanova³ Doctor of Biological Sciences, Researcher
¹Federal State Budgetary Educational Institution of Higher Education "Penza State Agrarian University", Russia, Penza
 ²Federal State Budgetary Educational Institution of Higher Education "Penza State University", Russia, Penza
 ³Institute of Biology and Immunology of Reproduction at the Bulgarian Academy of Sciences, Bulgaria, Sofia
tel. 8(8412)628359, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Recently, the demand for high-quality food has increased. Microgreens are a new functional product characterized by a high content of phytonutrients. Microgreens have been proven to contain a large number of biologically active compounds with antioxidant, antiviral, immune-stimulating and anti-diabetic effects. The purpose of the present study is to investigate the effect of phyto-stem cells of microgreens on the physiological and biochemical status of laboratory animals. Nine crops were initially selected for the study: common sunflower (Helianthus annuus), soya bean (Glicine hispida), common wheat (Triticum aestivum L.), oilseed rape (spring rape) (Brassica napus annua L.), common oat (Avéna satíva), field pea (Pisum sativum L.), lentil (Lens culinaris), barley (Hordéum vulgáre) and common flax (Línum usitatíssimum). In the selection process, the availability and cost of the seeds, the nutritional value of each crops and the content of the most important macro- and micronutrients were taken into account. For the experiment on laboratory animals, three crops were selected that were best suited for all the parameters under consideration - common wheat, common sunflower and oilseed rape. The results of the scientific experiment showed that the use of phyto-stem cells of microgreens in the diet of laboratory animals has a positive effect on hematological and biochemical parameters of the blood. In the group of animals that received oilseed rape and common sunflower microgreens, a decrease in blood platelets was observed, so these types of microgreens can be recommended to increase the amount of water in the peripheral blood. When analyzing the glucose level in these groups, a decrease in this indicator was also observed, which indicates the anti-diabetic effect of common sunflower and oilseed rape microgreens.
Keywords: microgreens, phyto-stem cells, laboratory rats, hematology, biochemistry.

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DOI 10.36461/NP.2023.67.3.012
UDC 574.23: 574.24: 577.2.01

PROLINE CONCENTRATION IN LEAVES IN ADVANCED HYBRID COMBINATIONS OF COTTON GENOTYPES (BC4F7) CREATED BY GENE PYRAMIDING UNDER THE INFLUENCE OF NaCl
Rakhmatova N.R., Imamkhodzhaeva A.S., Makamov A.H.,
Nomamatov I.S., Buriev Z.T.
Center of Genomics and Bioinformatics of the Academy of Sciences of the Republic of Uzbekistan, 2A Universitetskaya Str., 111215, Tashkent province, Uzbekistan This email address is being protected from spambots. You need JavaScript enabled to view it.

The article presents numerical indicators of proline changes that occurred in the leaves of seedlings of 25 lines of advanced cotton hybrids (BC4F7) as a result of a laboratory experiment that simulated salinization created by a solution of 200 mM NaCl. In the world literature, there are examples of a positive correlation between the accumulation of proline and plant stress, and it is also said that a stressful environment leads to overproduction of proline in plants, giving them stress resistance. As a result of the experiment, a high value of the proline content was observed in plants of six lines (BC4F7-2, BC4F7-3, BC4F7-14, BC4F7-17, BC4F7-21 and BC4F7-23) and ranged from 0.81±0.08 to 0.99±0.06 mg/g. The stress factor led to an increase in the accumulation of proline by 1,185 times (line BC4F7_7) up to 1,607 times (line BC4F7_14) compared to the control levels. The highest production and accumulation of proline was detected in the BC4F7_3 line (0.99±0.06 mg/g of dry leaf weight). That was 1.65 times higher than in the control version of the experiment. Based on the diagnosis obtained, some lines can be recommended as the primary breeding material to create salt-resistant lines.
Keywords: gene pyramiding, cotton, resistance, stress, salinity, proline.

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DOI 10.36461/NP.2023.67.3.011
UDC: 635.25

PRODUCTIVITY OF ONION HYBRIDS RELEASED IN THE ASTRAKHAN REGION
R.I. Dubin, Candidate of Agricultural Sciences
Tatishchev Astrakhan State University, Astrakhan, Russia, tel. 8(8512)24-66-52,
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

The aim of the research is to study the productivity of released onion hybrids, followed by the identification of the most promising ones with a complex of economically valuable features for their use in the Astrakhan Region. To achieve this goal, from 2020 to 2022, field studies were conducted at Agro-Progress Akhtuba LLC in the Akhtuba District of the Astrakhan Region, located in the northernmost part of the region. The selection of F1 hybrids for research gave an understanding of the absence of domestic samples released in the Astrakhan Region. Moreover, only F1 hybrids are represented among the objects of research, which does not allow their competitive comparison with varieties. Of all the studied F1 onion hybrids tested in the production conditions of the Astrakhan Region in 2020-2022, the F1 Meranto hybrid stood out. Its yield exceeded the control by 17.1 t/ha in 2020, by 17.9 t/ha in 2021, by 17.7 t/ha in 2022, and amounted to 78.6, 88.3 and 83.5 t/ha, respectively. In all the years of research, the highest level of profitability was observed on the hybrid F1 Meranto.
Keywords: released hybrids, onions, phenological stage, commercial yield, profitability.

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DOI 10.36461/NP.2023.67.3.010
UDC 633.63+631.82

THE EFFECT OF PRE-SOWING SEED TREATMENT WITH MICROBIOLOGICAL FERTILIZERS ON PRODUCTIVITY OF LENTILS
Yu.V. Koryagin, Candidate of Agricultural Sciences, Associate Professor;
V.V. Koshelyaev, Doctor of Agricultural Sciences, Professor;
O.N. Kukharev, Doctor of Engineering Sciences, Professor;
N.V. Koryagina, Candidate of Agricultural Sciences, Associate Professor
I.P. Koshelyaeva, Doctor of Agricultural Sciences, Associate Professor
Penza State Agrarian University, Russia, tel. 8(8412) 62-83-73, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

This article presents the results of studying the effect of pre-sowing treatment of lentil seeds with microbiological fertilizers. The issues of the influence of pre-sowing seed treatment with microbiological fertilizers on the growth power, the timing of interphase periods, plant survival and safekeeping, elements of productivity and grain yield are considered. The purpose of the research is to study and substantiate the inoculation of seeds with microbiological fertilizers as an independent technique in the technology of lentil cultivation, which ensures an increase in crop yield. As a result, it was found that inoculation of seeds with Azotovit, Phosphatovit and the mixture of both increases plant safety by 6.0...8.4% and plant survival by 7.0...9.6%. It has been shown that inoculation of seeds with microbiological fertilizers contributes to the formation of higher yields. The greatest increase in yield was obtained in the variant where the seeds were treated with a mixture of Azotovit and Phosphatovit – 0.38 t/ha. The increase in yield after inoculation of seeds with Azotovit and Phosphatovit separately was slightly lower and amounted to 0.31 and 0.34 t/ha. Thus, inoculation of seeds with microbiological fertilizers can be considered as an independent technique in the technology of lentil cultivation, which ensures an increase in crop yield.
Keywords: lentils, variety, microbiological fertilizers, vegetation period, growth power, grain yield.

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