DOI 10.36461/NP.2023.67.3.006
UDC 633.51 : 577.12

DIFFERENTIAL GENE EXPRESSION IN VARIOUS COTTON VARIETIES UNDER SALT STRESS CONDITIONS
V.S. Kamburova¹, Candidate of Biological Sciences, Head of Laboratory; A.S. Imamkhodzhaeva¹, Candidate of Biological Sciences, Head of Laboratory; I.B. Salakhutdinov¹, Candidate of Biological Sciences, Head of Laboratory; Bazarov D.K.^1,2, Junior Research Associate;
Sh.Kh. Mamatkulova¹, Junior Research Associate; O.L. Isomiddinova¹, Junior Research Associate; E.A. Latypova³, Candidate of Biological Sciences, Associate Professor
¹Center of Genomics and Bioinformatics of the Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan, tel. +998 99 846-41-02, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
²Digital Technologies and Artificial Intelligence Research Institute, Uzbekistan, Tashkent, Uzbekistan
³Federal State Budgetary Educational Institution of Higher Education "Penza State Agrarian University", Penza, Russia, tel. 8 (9680) 32-19-80, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Abstract. Soil salinization is one of the main problems of yield reduction in many crops, including cotton, in arid and semi-arid zones. At the same time, under conditions of salt stress in cotton, a change in the expression level of a number of genes determining both fiber development and yield, and cotton resistance to abiotic stresses is observed. However, currently there are no works on determining the level of gene expression in biotechnological cotton varieties under conditions of salinization. In this regard, the purpose of this work was to determine the level of gene expression in the biotechnological cotton variety Porlock-4 under model salt stress conditions. The results obtained showed that the expression level of almost all key genes, which determine fiber development and yield as well as the resistance of cotton to abiotic stress, decreased. The exceptions were the genes CYP707A (ABA 8'-hydroxylase) and LOX3.1 (linoleate 13S-lipoxygenase 3-1), the expression level of which increased. In addition, the results showed that the change in expression level was the most pronounced in the initial variety, Coker-312, while the variation in gene expression level in the variety Porlock-4 was the least. The analysis of the results indicates that the increased resistance of the Porlock-4 variety to salt stress is due to the activation of the phytohormone system (abscisic acid, brassinosteroids, auxins), the antioxidant system (enzymes and carotenoids), the increased synthesis of osmoprotectants (proline and water-soluble sugars) and unsaturated fatty acids. Higher yields and better fiber quality can be attributed to increased activity of the phytohormone system (abscisic acid, brassinosteroids, auxins) and photosynthesis, nitrogen and phosphorus metabolism as well as the synthesis of cell wall polysaccharides.
Keywords: cotton, salt stress, differential gene expression, phytohormones, antioxidant system, osmoprotectants.

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