UDC 575.167:633.853.59:631.527.51:001.891.55
DOI 10.36461/NP.2025.75.3.003

PCA BIPLOT AND STRESS INDICES FOR ANALYZING DROUGHT RESISTANCE OF INTERSPECIFIC RIL POPULATION
R.I. Mukhammadaliyev, Doctoral Student; A.Kh. Makamov (PhD), Head of Laboratory;
I.S. Normatov, Junior Researcher; U.A. Boykobilov, Junior Researcher;
N.N. Khusenov (PhD), Head of Laboratory; Zh.K. Norbekov (PhD), Senior Researcher;
A.B. Mamazhanov, Junior Researcher; M. M. Kholmuradova, Junior Researcher;
A. S. Imamkhodzhaeva (PhD), Head of Laboratory, Z.T. Buriev, Director
Center of Genomics and Bioinformatics. Academy of Sciences of the Republic of Uzbekistan,
Tashkent, Uzbekistan, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Current research deals with the drought resisitance of 80 RIL (recombinant inbred lines) cotton samples of the Gossypium tomentosum × G. mustelinum hybrid. Tests were carried out under optimal and simulated drought conditions, and fiber length, fiber yield (%), and thousand-seed weight were assessed using stress indices and principal component analysis (PCA Biplot). Statistical analysis revealed significant differences between the control and stress conditions. Genetic differences between the RIL lines were also observed. Based on the STI, SSI, and TOL indices, the following lines: RIL-67 (STI_FL = 1.14; SSI_FL = –5.25; TOL_FL = –4.33), RIL-34 (STI_LP = 1.10; TOL_LP = –0.35), and RIL-25 (SSI_SW = –2.94; TOL_SW = –3.12) were assessed as highly drought-resistant. The RIL-46, RIL-48, and RIL-60 lines showed stable yield under all conditions, low TOL (≤ ±1.0), and moderate STI. In contrast, lines such as RIL-06 (STI_FL = 0.86; SSI_FL = 5.11; TOL_FL = 4.87), RIL-58 (STI_SW = 0.81; SSI_SW = 8.19; TOL_SW = 20.52), and RIL-13 (STI_LP = 0.96; TOL_LP = 1.45) had a high level of stress sensitivity with negative effects detected. Principal component analysis (PCA Biplot) was used to explain 58.5% of the total variance based on the first two components and allowed to combine the lines according to their yield and stress response. Based on the research results, examined RIL lines were divided into three groups against their response to drought: tolerant (RIL-67, RIL-34, RIL-25), stable (RIL-46, RIL-48, RIL-60), and sensitive (RIL-06, RIL-58, RIL-13). These lines could be selected as promising samples for breeding programs.
Keywords: Gossypium tomentosum, Gossypium mustelinum, RIL, control conditions, drought resistance, PCA Biplot, stress indices.

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