DOI 10.36461/NP.2023.67.3.012
UDC 574.23: 574.24: 577.2.01

PROLINE CONCENTRATION IN LEAVES IN ADVANCED HYBRID COMBINATIONS OF COTTON GENOTYPES (BC4F7) CREATED BY GENE PYRAMIDING UNDER THE INFLUENCE OF NaCl
Rakhmatova N.R., Imamkhodzhaeva A.S., Makamov A.H.,
Nomamatov I.S., Buriev Z.T.
Center of Genomics and Bioinformatics of the Academy of Sciences of the Republic of Uzbekistan, 2A Universitetskaya Str., 111215, Tashkent province, Uzbekistan This email address is being protected from spambots. You need JavaScript enabled to view it.

The article presents numerical indicators of proline changes that occurred in the leaves of seedlings of 25 lines of advanced cotton hybrids (BC4F7) as a result of a laboratory experiment that simulated salinization created by a solution of 200 mM NaCl. In the world literature, there are examples of a positive correlation between the accumulation of proline and plant stress, and it is also said that a stressful environment leads to overproduction of proline in plants, giving them stress resistance. As a result of the experiment, a high value of the proline content was observed in plants of six lines (BC4F7-2, BC4F7-3, BC4F7-14, BC4F7-17, BC4F7-21 and BC4F7-23) and ranged from 0.81±0.08 to 0.99±0.06 mg/g. The stress factor led to an increase in the accumulation of proline by 1,185 times (line BC4F7_7) up to 1,607 times (line BC4F7_14) compared to the control levels. The highest production and accumulation of proline was detected in the BC4F7_3 line (0.99±0.06 mg/g of dry leaf weight). That was 1.65 times higher than in the control version of the experiment. Based on the diagnosis obtained, some lines can be recommended as the primary breeding material to create salt-resistant lines.
Keywords: gene pyramiding, cotton, resistance, stress, salinity, proline.

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