UDC 631:632:635.21
DOI 10.36461/NP.2023.67.3.002

POTATO RESISTANCE TO GLOBODERA ROSTOCHIENSIS AND GLOBODERA PALLIDA USING MOLECULAR MARKERS
I.O. Gazdanova, Candidate of Agricultural Sciences,
N.N. Doguzova, Postgraduate
Federal Scientific Center "Vladikavkaz Scientific Center of the Russian Academy of Sciences", Vladikavkaz, Russia e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it..

Studies were carried out to identify potato genotypes with resistance genes against the potato cyst nematodes using molecular markers. The detection of disease resistance genes using DNA markers can be an alternative approach to select potentially resistant samples. Potato (Solanum tuberosum L.) breeders widely use the resistance gene H1, which provides almost absolute resistance to the potato cyst nematode (Globodera rostochiensis) pathotype Ro1. The work was carried out in the Laboratory of Molecular Genetic Studies of Agricultural Plants of the Vladikavkaz Scientific Center of the Russian Academy of Sciences using the instrument line for PCR analysis. The breeding material was tested in advance under laboratory and field conditions for the most important economically valuable traits. Globodera rostochiensis and Globodera pallida are nematodes that cause serious damage to potato crops worldwide. They damage plants by sucking nutrients from the root tissue. The material consisted of potato leaves from 30 genotypes sampled during the vegetation period in the potato breeding nursery. Molecular markers of resistance genes were used to evaluate the potato samples: against the yellow potato cyst nematode – SCAR marker of the gene H1-TG 689, 57 R, STS marker Gro1-4-1 of the gene Gro1-4; against the pale potato cyst nematode – STS marker Gpa2-2 of the gene Gpa2. According to the results of molecular genetic analysis using DNA markers, potato genotypes that are gene carriers have been identified: Gro 1-4 – 14 samples, H1 – 20 samples, Gpa-2 –17. The article presents the results of the evaluation of the potato samples using the DNA marker-based evaluation of the resistant potato genotypes without the phenotypic evaluation.
Keywords: potato, gene, variety, hybrid, resistance, yellow potato cyst nematode, pale potato cyst nematode.

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