UDC 633.49:577.57
DOI 10.36461/NP.2023.66.2.006
GROWTH AND DEVELOPMENT UNDER IN VITRO CONDITIONS OF POTATO REGENERATE PLANTS OBTAINED BY SOMATIC EMBRYOGENESIS
F.I. Babadzhanova1, Research Assistant; Kh.A. Ubaydullayeva1, Doctor of Biological Sciences,
Head of Laboratory; V.S. Kamburova1, Candidate of Biological Sciences, Head of Laboratory;
E.A. Latypova2, Candidate of Biological Sciences, Associate Professor; A.A. Bolkiev1, Research Assistant;
S.A. Abdullaev1, Research Assistant; A.N. Abdullaev1, Research Assistant;
Z.T. Buriev1, Doctor of Biological Sciences, Head of Laboratory
1Center of Genomics and Bioinformatics Academy of Sciences the Republic of Uzbekistan, Tashkent, Uzbekistan, tel. +998 90 959-93-84, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.;
2Federal State Budgetary Educational Institution of Higher Education "Penza State Agrarian University", Penza, Russia, tel. 8 (9680) 32-19-80, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Potato is the main food crop both in the world and in Uzbekistan. However, abiotic and biotic stresses lead to lower potato yields To solve these problems, new varieties must be obtained using modern methods of genomics and biotechnology, which is not possible without micropropagation. Phytohormones play an important role in the process of potato micropropagation. Due to the great influence of hormones on the processes of plant development, their role in the stages of potato growth and development has been studied. Our study summarizes the effects of the phytohormones kinetin and 6-benzylaminopurine (BAP) on the growth and development of new biotech potato lines generated by RNA interference (RNAi) technology of the phytochrome B (phyB) gene during somatic embryogenesis. Phytochrome B is a plant photoreceptor for red/far-red light. In potato, it mediates photoperiodic control of tuber formation in a negative regulatory mechanism by inhibiting its initial stages. Consequently, suppression of phyB expression by RNAi could lead to an increase in tuber number. The effects of different concentrations of kinetin and BAP on plant growth, number of nodes, and formation and development of the root system of potato RNAi lines during somatic embryogenesis in micropropagation were studied. The highest plant growth and development were obtained when a growth medium containing 1.0 mg/L kinetin was used. This combination increased plant height in all biotech lines and contributed to more efficient root system formation.
Keywords: potato, in vitro, kinetin, 6-benzylaminopurine (BAP), Murashige and Skoog, micropropagation.
